Botanical Chlorophyll Paper Chromatography Extract

45 mins Ages 7-9 Indoor

An engaging, screen-free learning activity that builds real-world creativity and critical thinking for kids aged 7 to 9.

Materials Needed

  • • Fresh baby spinach leaves (3–4 leaves)
  • • Rubbing alcohol (70% isopropyl) (1/4 cup)
  • • White paper coffee filter (1 filter)
  • • Heavy coin (quarter or large token) (1 unit)
  • • Tall small glass jar (1 unit)
  • • Pencil and tape (1 set)

Pro Tips

  • → Use the ribbed edge of a coin to firmly roll and crush fresh spinach leaves directly onto the paper filter 2 cm from the bottom.
  • → Repeat the coin rolling 10 times over the exact same spot to build up a dark, concentrated green pigment bar.
  • → Suspend the strip so only the very bottom touches the alcohol; the green line must remain above the liquid surface.

Safety First

Adult handles rubbing alcohol; use in a well-ventilated room away from open flames.

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Step-by-Step Instructions

1

Step 1

Cut a 3-centimeter-wide by 15-centimeter-long strip from a white paper coffee filter.

2

Step 2

Place a dark spinach leaf over the strip 2 cm from the bottom and roll the ribbed edge of a coin over it 10 times to transfer a concentrated green stripe.

3

Step 3

Pour 1 cm of rubbing alcohol into a small glass jar and suspend the paper strip vertically from a pencil resting across the rim.

4

Step 4

Watch the alcohol travel up the paper over 20 minutes, separating the plant pigments into distinct bands of yellow, olive green, and bright blue-green.

What they are learning

Photosynthetic pigment fractionation (Chlorophyll A, B, Carotenoids) Capillary solvent migration Plant biochemistry